Ex vivo immune profiling in patient blood enables quantification of innate immune effector functions.

ABSTRACT: The assessment of a patient's immune function is critical in many clinical situations. In complex clinical immune dysfunction like sepsis, which results from a loss of immune homeostasis due to microbial infection, a plethora of pro- and anti-inflammatory stimuli may occur consecutively or simultaneously. Thus, any immunomodulatory therapy would require in-depth knowledge of an individual patient's immune status at a given time. Whereas lab-based immune profiling often relies solely on quantification of cell numbers, we used an ex vivo whole-blood infection model in combination with biomathematical modeling to quantify functional parameters of innate immune cells in blood from patients undergoing cardiac surgery. These patients experience a well-characterized inflammatory insult, which res

SUBMITTER: Lehnert T 

PROVIDER: S-EPMC8187451 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature