Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production.

ABSTRACT: Recent evidence supports involvement of the acute phase protein haptoglobin in numerous events during mammalian reproduction. The present study represents an in-depth investigation of haptoglobin expression and secretion in the porcine oviduct and uterus, and assesses its effect on porcine in vitro embryo production. A systematic study was made of sows in different oestrous stages: late follicular, early luteal and late luteal stages. Relative haptoglobin mRNA abundance was quantified by RT-qPCR. In addition, expression of the protein was analysed by immunohistochemistry and the results were complemented by Western-blot and proteomic analyses of the oviductal and uterine fluids. In vitro porcine fertilization and embryo culture were carried out in the presence of haptoglobin. The results i

SUBMITTER: Garcia-Vazquez FA 

PROVIDER: S-EPMC8187724 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature