Identification of genetic modifiers of lifespan on a high sugar diet in the Drosophila Genetic Reference Panel.

ABSTRACT: Genome-wide association studies (GWAS) have become beneficial in identifying genetic variants underlying susceptibility to various complex diseases and conditions, including obesity. Utilizing the Drosophila Genetic Reference Panel (DGRP), we performed a GWAS of lifespan of 193 genetically distinct lines on a high sugar diet (HSD). The DGRP analysis pipeline determined the most significant lifespan associated polymorphisms were within loci of genes involved in: neural processes, behavior, development, and apoptosis, among other functions. Next, based on the relevance to obesity pathology, and the availability of transgenic RNAi lines targeting the genes we identified, whole-body in vivo knockdown of several candidate genes was performed. We utilized the GAL4-UAS binary expres

SUBMITTER: Patel SP 

PROVIDER: S-EPMC8187823 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature