Ontology highlight
ABSTRACT:
SUBMITTER: McCabe MC
PROVIDER: S-EPMC8188056 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
McCabe Maxwell C MC Schmitt Lauren R LR Hill Ryan C RC Dzieciatkowska Monika M Maslanka Mark M Daamen Willeke F WF van Kuppevelt Toin H TH Hof Danique J DJ Hansen Kirk C KC
Molecular & cellular proteomics : MCP 20210603
The extracellular matrix is a key component of tissues, yet it is underrepresented in proteomic datasets. Identification and evaluation of proteins in the extracellular matrix (ECM) has proved challenging due to the insolubility of many ECM proteins in traditional protein extraction buffers. Here we separate the decellularization and ECM extraction steps of several prominent methods for evaluation under real-world conditions. The results are used to optimize a two-fraction ECM extraction method. ...[more]