Transcription shapes DNA replication initiation to preserve genome integrity.

ABSTRACT:

Background

Early DNA replication occurs within actively transcribed chromatin compartments in mammalian cells, raising the immediate question of how early DNA replication coordinates with transcription to avoid collisions and DNA damage.

Results

We develop a high-throughput nucleoside analog incorporation sequencing assay and identify thousands of early replication initiation zones in both mouse and human cells. The identified early replication initiation zones fall in open chromatin compartments and are mutually exclusive with transcription elongation. Of note, early replication initiation zones are mainly located in non-transcribed regions adjacent to transcribed regions. Mechanistically, we find that RNA polymerase II actively redistributes the chromatin-bound mini-chromo

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8188667 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature