Lipid-Bicelle-Coated Microfluidics for Intracellular Delivery with Reduced Fouling.

ABSTRACT: Innovative technologies for intracellular delivery are ushering in a new era for gene editing, enabling the utilization of a patient's own cells for stem cell and immunotherapies. In particular, cell-squeezing platforms provide unconventional forms of intracellular delivery, deforming cells through microfluidic constrictions to generate transient pores and to enable effective diffusion of biomolecular cargo. While these devices are promising gene-editing platforms, they require frequent maintenance due to the accumulation of cellular debris, limiting their potential for reaching the throughputs necessary for scalable cellular therapies. As these cell-squeezing technologies are improved, there is a need to develop next-generation platforms with higher throughput and longer lifespan, importa

SUBMITTER: Belling JN 

PROVIDER: S-EPMC8188960 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature