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Characterization and phylogenetic analysis of the complete chloroplast genome sequence of Pertya multiflora (Asteraceae), a rare and endangered wild plant species in China.

ABSTRACT: Pertya multiflora (Asteraceae) is a rare wild plant species narrowly distributed in Zhejiang province, China. In our present study, we assembled its complete chloroplast genome using high-throughput sequencing data. The results indicated that the whole chloroplast genome of P. multiflora was 153,396 bp in length. Its large single copy, small single copy, and inverted region sequences were 84,575 bp, 18,451 bp, and 25,185 bp. The P. multiflora chloroplast genome was composed of 134 genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes, and two pseudogenes. Phylogenetic analysis results showed that P. multiflora was grouped with Gerbera jamesonii, with 100% bootstrap support.

SUBMITTER: Liu Y

PROVIDER: S-EPMC8189106 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Characterization and phylogenetic analysis of the complete chloroplast genome sequence of Pertya multiflora (Asteraceae), a rare and endangered wild plant species in China.

Liu Ye Y Zhang Huijuan H Wu Qian Q Huang Wenxin W Wu Tao T Guan Ming M Jiang Ming M

Mitochondrial DNA. Part B, Resources 20210607 7

Pertya multiflora (Asteraceae) is a rare wild plant species narrowly distributed in Zhejiang province, China. In our present study, we assembled its complete chloroplast genome using high-throughput sequencing data. The results indicated that the whole chloroplast genome of P. multiflora was 153,396 bp in length. Its large single copy, small single copy, and inverted region sequences were 84,575 bp, 18,451 bp, and 25,185 bp. The P. multiflora chloroplast genome was composed ...[more]

PMID: 34151020

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Project description:Dendropanax oligodontus, which belongs to the family Araliaceae, is an endemic and endangered species of Hainan Island, China. It has potential economic and medicinal value owing to the presence of phenylpropanoids, flavonoids, triterpenoids, etc. The analysis of the structure and characteristics of the D. oligodontus chloroplast genome (cpDNA) is crucial for understanding the genetic and phylogenetic evolution of this species. In this study, the cpDNA of D. oligodontus was sequenced for the first time using next-generation sequencing methods, assembled, and annotated. We observed a circular quadripartite structure comprising a large single-copy region (86,440 bp), a small single-copy region (18,075 bp), and a pair of inverted repeat regions (25,944 bp). The total length of the cpDNA was 156,403 bp, and the GC% was 37.99%. We found that the D. oligodontus chloroplast genome comprised 131 genes, with 86 protein-coding genes, 8 rRNA genes, and 37 tRNAs. Furthermore, we identified 26,514 codons, 13 repetitive sequences, and 43 simple sequence repeat sites in the D. oligodontus cpDNA. The most common amino acid encoded was leucine, with a strong A/T preference at the third position of the codon. The prediction of RNA editing sites in the protein-coding genes indicated that RNA editing was observed in 19 genes with a total of 54 editing sites, all of which involved C-to-T transitions. Finally, the cpDNA of 11 species of the family Araliaceae were selected for comparative analysis. The sequences of the untranslated regions and coding regions among 11 species were highly conserved, and minor differences were observed in the length of the inverted repeat regions; therefore, the cpDNAs were relatively stable and consistent among these 11 species. The variable hotspots in the genome included clpP, ycf1, rnK-rps16, rps16-trnQ, atpH-atpI, trnE-trnT, psbM-trnD, ycf3-trnS, and rpl32-trnL, providing valuable molecular markers for species authentication and regions for inferring phylogenetic relationships among them, as well as for evolutionary studies. Evolutionary selection pressure analysis indicated that the atpF gene was strongly subjected to positive environmental selection. Phylogenetic analysis indicated that D. oligodontus and Dendropanax dentiger were the most closely related species within the genus, and D. oligodontus was closely related to the genera Kalopanax and Metapanax in the Araliaceae family. Overall, the cp genomes reported in this study will provide resources for studying the genetic diversity and conservation of the endangered plant D. oligodontus, as well as resolving phylogenetic relationships within the family.

Dataset Information

Characterization and phylogenetic analysis of the complete chloroplast genome sequence of Pertya multiflora (Asteraceae), a rare and endangered wild plant species in China.

Publications

Characterization and phylogenetic analysis of the complete chloroplast genome sequence of <i>Pertya multiflora</i> (Asteraceae), a rare and endangered wild plant species in China.

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