Cell-type specific analysis of physiological action of estrogen in mouse oviducts.

ABSTRACT: One of the endogenous estrogens, 17β-estradiol (E₂ ) is a female steroid hormone secreted from the ovary. It is well established that E₂ causes biochemical and histological changes in the uterus. However, it is not completely understood how E₂ regulates the oviductal environment in vivo. In this study, we assessed the effect of E₂ on each oviductal cell type, using an ovariectomized-hormone-replacement mouse model, single-cell RNA-sequencing (scRNA-seq), in situ hybridization, and cell-type-specific deletion in mice. We found that each cell type in the oviduct responded to E₂ distinctively, especially ciliated and secretory epithelial cells. The treatment of exogenous E₂ did not drastically alter the transcriptomic profile f

SUBMITTER: McGlade EA 

PROVIDER: S-EPMC8189321 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature