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Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.

ABSTRACT: This study provides transcriptomic characterization of the cells of the crista ampullaris, sensory structures at the base of the semicircular canals that are critical for vestibular function. We performed single-cell RNA-seq on ampullae microdissected from E16, E18, P3, and P7 mice. Cluster analysis identified the hair cells, support cells and glia of the crista as well as dark cells and other nonsensory epithelial cells of the ampulla, mesenchymal cells, vascular cells, macrophages, and melanocytes. Cluster-specific expression of genes predicted their spatially restricted domains of gene expression in the crista and ampulla. Analysis of cellular proportions across developmental time showed dynamics in cellular composition. The new cell types revealed by single-cell RNA-seq could be important for understanding crista function and the markers identified in this study will enable the examination of their dynamics during development and disease.

SUBMITTER: Wilkerson BA 

PROVIDER: S-EPMC8189719 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.

Wilkerson Brent A BA   Zebroski Heather L HL   Finkbeiner Connor R CR   Chitsazan Alex D AD   Beach Kylie E KE   Sen Nilasha N   Zhang Renee C RC   Bermingham-McDonogh Olivia O  

eLife 20210518

This study provides transcriptomic characterization of the cells of the crista ampullaris, sensory structures at the base of the semicircular canals that are critical for vestibular function. We performed single-cell RNA-seq on ampullae microdissected from E16, E18, P3, and P7 mice. Cluster analysis identified the hair cells, support cells and glia of the crista as well as dark cells and other nonsensory epithelial cells of the ampulla, mesenchymal cells, vascular cells, macrophages, and melanoc  ...[more]

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