Follicular helper-T cells restore CD8⁺-dependent antitumor immunity and anti-PD-L1/PD-1 efficacy.

ABSTRACT:

Background

T follicular helper cells (Tfh) are essential to shape B cell response during germinal center formation. Tfh accumulation has been reported in various human cancers, with positive or negative prognostic roles. However, the mechanisms explaining the accumulation of Tfh and their role in cancer remain obscure.

Methods

In vitro differentiated and mouse cell sorted Tfh phenotype was evaluated by flow cytometry and quantitative PCR (qPCR). Antitumor effect of Tfh was evaluated by adoptive transfer in different tumor-bearing mice models. The involvement of immune cells, cytokines and chemokines was evaluated, using depleting antibodies. Chemokines and cytokines expression and production were evaluated by qPCR and ELISA. In human, the impact of immune cells and chemokine

SUBMITTER: Niogret J 

PROVIDER: S-EPMC8190041 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature