Real-Time insight into in vivo redox status utilizing hyperpolarized [1-¹³C] N-acetyl cysteine.

ABSTRACT: Drastic sensitivity enhancement of dynamic nuclear polarization is becoming an increasingly critical methodology to monitor real-time metabolic and physiological information in chemistry, biochemistry, and biomedicine. However, the limited number of available hyperpolarized ¹³C probes, which can effectively interrogate crucial metabolic activities, remains one of the major bottlenecks in this growing field. Here, we demonstrate [1-¹³C] N-acetyl cysteine (NAC) as a novel probe for hyperpolarized ¹³C MRI to monitor glutathione redox chemistry, which plays a central part of metabolic chemistry and strongly influences various therapies. NAC forms a disulfide bond in the presence of reduced glutathione, which generates a spectroscopically detectable product that

SUBMITTER: Yamamoto K 

PROVIDER: S-EPMC8190077 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature