A high-throughput microfluidic bilayer co-culture platform to study endothelial-pericyte interactions.

ABSTRACT: Microphysiological organ-on-chip models offer the potential to improve the prediction of drug safety and efficacy through recapitulation of human physiological responses. The importance of including multiple cell types within tissue models has been well documented. However, the study of cell interactions in vitro can be limited by complexity of the tissue model and throughput of current culture systems. Here, we describe the development of a co-culture microvascular model and relevant assays in a high-throughput thermoplastic organ-on-chip platform, PREDICT96. The system consists of 96 arrayed bilayer microfluidic devices containing retinal microvascular endothelial cells and pericytes cultured on opposing sides of a microporous membrane. Compatibility of the PREDICT96 platform with a vari

SUBMITTER: Rogers MT 

PROVIDER: S-EPMC8190127 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature