CircMELK promotes glioblastoma multiforme cell tumorigenesis through the miR-593/EphB2 axis.

ABSTRACT: A number of studies indicate that circular RNAs (circRNAs) play paramount roles in regulating the biological behavior of glioblastoma multiforme (GBM). In this study, we investigated the underlying mechanism of circMELK in GBM. Real-time PCRs were used to examine the expression of circMELK in glioma tissues and normal brain tissues (NBTs). Localization of circMELK in GBM cells was estimated by fluorescence in situ hybridization (FISH). Transwell migration and three-dimensional invasion assays were performed to examine glioma cell migration and invasion in vitro. Spheroid formation, clonogenicity, and cell viability assays were implemented to test the stemness of glioma stem cells (GSCs). The functions of circMELK in vivo were investigated in a xenograft nude-mouse mode

SUBMITTER: Zhou F 

PROVIDER: S-EPMC8190146 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature