Development of a time-series shotgun metagenomics database for monitoring microbial communities at the Pacific coast of Japan.

ABSTRACT: Although numerous metagenome, amplicon sequencing-based studies have been conducted to date to characterize marine microbial communities, relatively few have employed full metagenome shotgun sequencing to obtain a broader picture of the functional features of these marine microbial communities. Moreover, most of these studies only performed sporadic sampling, which is insufficient to understand an ecosystem comprehensively. In this study, we regularly conducted seawater sampling along the northeastern Pacific coast of Japan between March 2012 and May 2016. We collected 213 seawater samples and prepared size-based fractions to generate 454 subsets of samples for shotgun metagenome sequencing and analysis. We also determined the sequences of 16S rRNA (n = 111) and 18S rRNA (n = 47) gene ampl

SUBMITTER: Yoshitake K 

PROVIDER: S-EPMC8190148 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature