Chromosome compartments on the inactive X guide TAD formation independently of transcription during X-reactivation.

ABSTRACT: A hallmark of chromosome organization is the partition into transcriptionally active A and repressed B compartments, and into topologically associating domains (TADs). Both structures were regarded to be absent from the inactive mouse X chromosome, but to be re-established with transcriptional reactivation and chromatin opening during X-reactivation. Here, we combine a tailor-made mouse iPSC reprogramming system and high-resolution Hi-C to produce a time course combining gene reactivation, chromatin opening and chromosome topology during X-reactivation. Contrary to previous observations, we observe A/B-like compartments on the inactive X harbouring multiple subcompartments. While partial X-reactivation initiates within a compartment rich in X-inactivation escapees, it then occurs rapidly a

SUBMITTER: Bauer M 

PROVIDER: S-EPMC8190187 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature