Dataset Information

Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence.

ABSTRACT: The routine evaluation of water environments is necessary to manage enteric virus-mediated fecal contamination and the possible emergence of novel variants. Here, we detected human rotavirus A (HRVA) circulating in two wastewater treatment plants, two lakes, irrigation water and a wastewater landfill located in Riyadh. VP7-derived surface protein sequences were assessed by phylogenetic analyses and inspection of thermotolerance-mediated secondary structure and seasonal variation. HRVA was most prevalent at An-Nazim wastewater landfill (AN-WWLF; 63.89%). Phylogenetic analyzes revealed the predominance of HRVA G2 lineage for the first time in Saudi Arabia. Moreover, a single HRVA sequence (2B64I-ANLF3/2018) was recovered at 45 °C from AN-WWLF; secondary structure prediction indicated that this sequence was thermotolerant with a high hydrophobicity, an absence of Ramachandran outliers, and a higher content of proline patches on the protein surface. Varied relationships were significantly observed between sampling areas influenced by temperature ranges (p < 0.05). HRVA prevalence was influenced by seasonal variations, favoring moderate temperatures in late autumn and early winter in all locations. However, a significant temperature impact was detected in Wadi-Hanifah Lake (p = 0.01). Our study extends the knowledge of currently circulating HRVA genotypes, and indicates the probable emergence of thermotolerant strains and seasonally mediated HRVA prevalence.

SUBMITTER: Nour I

PROVIDER: S-EPMC8190275 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence.

Nour Islam I Hanif Atif A Alanazi Ibrahim O IO Al-Ashkar Ibrahim I Alhetheel Abdulkarim A Eifan Saleh S

Scientific reports 20210609 1

The routine evaluation of water environments is necessary to manage enteric virus-mediated fecal contamination and the possible emergence of novel variants. Here, we detected human rotavirus A (HRVA) circulating in two wastewater treatment plants, two lakes, irrigation water and a wastewater landfill located in Riyadh. VP7-derived surface protein sequences were assessed by phylogenetic analyses and inspection of thermotolerance-mediated secondary structure and seasonal variation. HRVA was most p ...[more]

PMID: 34108565

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Project description:BackgroundChildren diagnosed with autism spectrum disorder (ASD) have social and cognitive disabilities. For parents, these behaviors can lead to humiliation, social exclusion, and isolation. Stigma is a problem that not only may affect the individual with high functioning autism, but has the potential to extend to their family as well. Indeed, research indicates that the parents of children with disabilities (including ASD) commonly experience stigmatizing reactions from others.Aim of workTo determine the prevalence of self and enacted stigma among parents of ASD children from Riyadh, Saudi Arabia, and recognize possible risk factors and causes of stigmatization.Material and methodsAn observational cross-sectional study involving the parents of ASD children from Riyadh, Saudi Arabia. About 163 participants were included from the Centre for Autism Research (CFAR) at King Faisal Specialist Hospital & Research Centre (KFSH & RC) in Riyadh. The primary material used for data collection was a five-point scale questionnaire developed by the investigator. The questionnaire consisted closed ended questions related to the participants' demographic data, autistic child, family life, resources, and social experiences as a parent. The collected data were analyzed using descriptive statistics and appropriate statistical analysis, using Statistical package for the social sciences (SPSS), version 22. The level of significance was P value < .05.ResultsThe findings of this work indicated that 55 (33.7%) of the parents scored more than 3 in answering questions related to felt stigma; 82 (50.3%) were fathers. On the other hand, 108 (66.3%) did not feel stigmatized being a parent of autistic child. Forty-two (25.8%) of the parents scored more than 3 in answering questions related to enacted stigma, whereas 121 (74.2%) did not face enacted stigmatization being a parent of autistic child. There was a strong tendency for mothers (41, 75%) to feel more self- and enacted stigmatized than fathers (P < .05). Moreover, the gender of the autistic child, consanguinity, severity of the ASD, and the monthly income of the family showed no significant difference in the parents who scored more than 3 for felt- or enacted stigmatization (P > .05).ConclusionAmong participated parents of children with ASD in Riyadh, Saudi Arabia, 33.7% of them experienced stigmatization, and mothers felt more self- and enacted stigmatized when compared to fathers. Public awareness among people in Saudi Arabia have been increased and, thus, negative stigmatization has decreased among parents in the country. A high level of felt stigma suggests the need for psychological intervention for the family to help cope with this new situation. A higher level of enacted stigma suggests the need for their rights (individuals with ASD) and increased awareness in the society. It is recommended that further research is needed in different centers in Riyadh and other regions of Saudi Arabia to investigate the prevalence of stigma in parents of autistic children.

Project description:Background: Disordered eating among athletes and physical activity practitioners is a growing concern that can negatively impact health and performance. Understanding the prevalence and predictors of disordered eating in these populations is essential for developing effective interventions. Objective: To investigate disordered eating tendencies among athletes and physical activity practitioners in Riyadh, Saudi Arabia. Methods: Participants from five athletic clubs and six fitness centers were surveyed. Data were collected using an interviewer-administered questionnaire that included the Eating Attitudes Test (EAT-26), the Abridged Nutrition for Sport Knowledge Questionnaire (ANSKQ), and the Global Physical Activity Questionnaire (GPAQ). Anthropometric data were also collected. The study comprised a sample of 263 individuals, who were divided into two groups: athletes (n = 121) and physical activity practitioners (n = 142). Results: The average age of the participants was 26.41 years with a standard deviation of 8.1 years. Females comprised nearly two thirds of the sample, representing 60.8% of the participants. Physical activity practitioners exhibited higher disordered eating scores compared to athletes. The total EAT-26 scores were significantly higher in practitioners (15.63 ± 4.12) than in athletes (13.21 ± 3.89; p < 0.001). Subscale scores for Dieting (8.95 ± 2.73 vs. 7.49 ± 2.58; p = 0.001) and Bulimia (3.32 ± 1.15 vs. 2.20 ± 1.03; p < 0.001) were also higher in practitioners. BMI was a significant predictor of higher total EAT-26 scores and its subsets, indicating a greater risk of disordered eating behaviors in individuals with higher BMI. Participants with lower levels of sports nutritional knowledge and those engaging in low-intensity physical activities were more likely to exhibit disordered eating tendencies. Conclusions: The findings highlight that physical activity practitioners are at a higher risk of disordered eating compared to athletes. Addressing BMI and improving nutritional knowledge are key strategies for preventing and managing disordered eating behaviors in physically active populations. Interventions targeting these areas may enhance health outcomes and performance among athletes and practitioners.

Dataset Information

Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence.

Publications

Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence.

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