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Generation and validation of APOE knockout human iPSC-derived cerebral organoids.

ABSTRACT: Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE^-/- ) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE^-/- cerebral organoids can serve as a useful tool to study apoE functions and apoE-related pathogenic mechanisms in AD. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).

SUBMITTER: Martens YA

PROVIDER: S-EPMC8190508 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Generation and validation of APOE knockout human iPSC-derived cerebral organoids.

Martens Yuka A YA Xu Siming S Tait Richard R Li Gary G Zhao Xinping C XC Lu Wenyan W Liu Chia-Chen CC Kanekiyo Takahisa T Bu Guojun G Zhao Jing J

STAR protocols 20210605 2

Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE-/- ) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE-/- cerebral organoids can ...[more]

PMID: 34151296

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