Hematopoietic stem cells retain functional potential and molecular identity in hibernation cultures.

ABSTRACT: Advances in the isolation and gene expression profiling of single hematopoietic stem cells (HSCs) have permitted in-depth resolution of their molecular program. However, long-term HSCs can only be isolated to near purity from adult mouse bone marrow, thereby precluding studies of their molecular program in different physiological states. Here, we describe a powerful 7-day HSC hibernation culture system that maintains HSCs as single cells in the absence of a physical niche. Single hibernating HSCs retain full functional potential compared with freshly isolated HSCs with respect to colony-forming capacity and transplantation into primary and secondary recipients. Comparison of hibernating HSC molecular profiles to their freshly isolated counterparts showed a striking degree of molecular simi

SUBMITTER: Oedekoven CA 

PROVIDER: S-EPMC8190576 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature