Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half-lives.

ABSTRACT:

Background

Interleukin-15 (IL-15) is a critical cytokine for the development, proliferation, and function of natural killer (NK) cells, NKT cells, and CD8⁺ memory T cells and has become one of the most promising protein molecules for the treatment of cancer and viral diseases. However, there are several limitations in applying IL-15 in therapy, such as its low yield in vitro, limited potency, and short half-life in vivo. To date, there are several recombinant IL-15 agonists based on configurational modifications that are being pursued in the treatment of cancer, such as ALT-803, which are mainly produced from mammalian cells.

Results

In this study, we designed two different forms of the IL-15 complex, which were formed by the noncovalent assembly of IL-15 with dim

SUBMITTER: Xu H 

PROVIDER: S-EPMC8190845 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature