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An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells.

ABSTRACT: Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.

SUBMITTER: Baglia RA

PROVIDER: S-EPMC8190907 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells.

Baglia Regina A RA Mills Kira R KR Mitra Koushambi K Tutol Jasmine N JN Ball Darby D Page Kierstin M KM Kallu Jyothi J Gottipolu Sriharika S D'Arcy Sheena S Nielsen Steven O SO Dodani Sheel C SC

RSC chemical biology 20210315 3

Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, <i>in vitro</i> enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform. ...[more]

PMID: 34212150

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