The Wnt-specific astacin proteinase HAS-7 restricts head organizer formation in Hydra.

ABSTRACT:

Background

The Hydra head organizer acts as a signaling center that initiates and maintains the primary body axis in steady state polyps and during budding or regeneration. Wnt/beta-Catenin signaling functions as a primary cue controlling this process, but how Wnt ligand activity is locally restricted at the protein level is poorly understood. Here we report a proteomic analysis of Hydra head tissue leading to the identification of an astacin family proteinase as a Wnt processing factor.

Results

Hydra astacin-7 (HAS-7) is expressed from gland cells as an apical-distal gradient in the body column, peaking close beneath the tentacle zone. HAS-7 siRNA knockdown abrogates HyWnt3 proteolysis in the head tissue and induces a robust double axis phenotype, which is rescued by simult

SUBMITTER: Ziegler B 

PROVIDER: S-EPMC8191133 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature