Generation of an isoform-level transcriptome atlas of macrophage activation.

ABSTRACT: RNA-seq is routinely used to measure gene expression changes in response to cell perturbation. Genes upregulated or downregulated following some perturbation are designated as genes of interest, and their most expressed isoform(s) would then be selected for follow-up experimentation. However, because of its need to fragment RNA molecules, RNA-seq is limited in its ability to capture gene isoforms and their expression patterns. This lack of isoform-specific data means that isoforms would be selected based on annotation databases that are incomplete, not tissue specific, or do not provide key information on expression levels. As a result, minority or nonexistent isoforms might be selected for follow-up, leading to loss in valuable resources and time. There is therefore a great need to compre

SUBMITTER: Vollmers AC 

PROVIDER: S-EPMC8191339 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature