Identification of multiple substrate binding sites in SLC4 transporters in the outward-facing conformation: Insights into the transport mechanism.

ABSTRACT: Solute carrier family 4 (SLC4) transporters mediate the transmembrane transport of HCO₃^-, CO₃^2-, and Cl^- necessary for pH regulation, transepithelial H⁺/base transport, and ion homeostasis. Substrate transport with varying stoichiometry and specificity is achieved through an exchange mechanism and/or through coupling of the uptake of anionic substrates to typically co-transported Na⁺. Recently solved outward-facing structures of two SLC4 members (human anion exchanger 1 [hAE1] and human electrogenic sodium bicarbonate cotransporter 1 [hNBCe1]) with different transport modes (Cl^-/HCO₃^- exchange versus Na⁺-CO₃^2- symport) revealed highly conserved thre

SUBMITTER: Zhekova HR 

PROVIDER: S-EPMC8191340 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature