Differential Expression of CD43, CD81, and CD200 in Classic Versus Variant Hairy Cell Leukemia.

ABSTRACT:

Background

Hairy cell leukemia (HCL) and hairy cell leukemia variant (HCLv) are rare diseases with overlapping clinicopathological features. Features distinguishing HCL from HCLv include expression of CD25, CD123, CD200, annexin-A1, and the presence of BRAF V600E mutation. HCLv typically lacks these markers, but they may occur in a subgroup of HCL patients with an aggressive clinical course. We examined CD43, CD81, CD79b, and CD200 expression in HCL and HCLv.

Methods

Multiparametric flow cytometry (FCM) was performed on blood from 59 HCL and 15 HCLv patients for protocol entry. Mean fluorescent intensity (MFI) of CD43, CD79b, CD81, and CD200 was determined (for CD200, n = 17 and 7, respectively).

Results

Median MFI of HCL vs HCLv was 545 vs 272 for CD43, 602 vs 2,450

SUBMITTER: Salem DA 

PROVIDER: S-EPMC8191384 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature