Synthetic Antibody Mimics Based on Cancer-Targeting Immunostimulatory Peptides.

ABSTRACT: De novo cancer-targeting immunostimulatory peptides have been designed and developed as synthetic antibody mimics. A series of bifunctional peptides incorporating NKp30-binding and NK-cell-activating domains were synthesized as linear dimers and then extended into branching trimeric peptides by the incorporation of GRP78-targeting and tumor-cell-binding sequences. A selected trimeric peptide from this small set of peptides displayed binding capabilities on GRP78⁺ HepG2 and A549 target cells. Cell binding diminished in the presence of an anti-GRP78 peptide blocker, thus suggesting GRP78-binding dependence. Similarly, the selected trimeric peptide was also found to exhibit NK cell binding in an NKp30-dependent manner, which translated into NK cell activation as indicated by cytoki

SUBMITTER: Descalzi-Montoya D 

PROVIDER: S-EPMC8191480 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature