A structural intermediate pre-organizes the add adenine riboswitch for ligand recognition.

ABSTRACT: Riboswitches are RNA sequences that regulate gene expression by undergoing structural changes upon the specific binding of cellular metabolites. Crystal structures of purine-sensing riboswitches have revealed an intricate network of interactions surrounding the ligand in the bound complex. The mechanistic details about how the aptamer folding pathway is involved in the formation of the metabolite binding site have been previously shown to be highly important for the riboswitch regulatory activity. Here, a combination of single-molecule FRET and SHAPE assays have been used to characterize the folding pathway of the adenine riboswitch from Vibrio vulnificus. Experimental evidences suggest a folding process characterized by the presence of a structural intermediate involved in ligand recognit

SUBMITTER: St-Pierre P 

PROVIDER: S-EPMC8191784 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature