Ontology highlight
ABSTRACT:
SUBMITTER: Li W
PROVIDER: S-EPMC8192075 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Li Wenqing W Chan Chun C Zeng Chunxi C Turk Rolf R Behlke Mark A MA Cheng Xiaolin X Dong Yizhou Y
Bioconjugate chemistry 20200306 3
CRISPR-Cas12a, a type-V CRISPR-Cas endonuclease, is an effective genome editing platform. To improve the gene editing efficiency of Cas12a, we rationally designed small molecule enhancers through a combined computational approach. First, we used extensive molecular dynamics (MD) simulations to explore the conformational landscape of Cas12a from <i>Acidaminococcus</i> (AsCas12a), revealing distinct conformational states that could be targeted by small molecules to modulate its genome editing func ...[more]