Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis.

ABSTRACT: RNA abundance is generally sensitive to perturbations in decay and synthesis rates, but crosstalk between RNA polymerase II transcription and cytoplasmic mRNA degradation often leads to compensatory changes in gene expression. Here, we reveal that widespread mRNA decay during early apoptosis represses RNAPII transcription, indicative of positive (rather than compensatory) feedback. This repression requires active cytoplasmic mRNA degradation, which leads to impaired recruitment of components of the transcription preinitiation complex to promoter DNA. Importin α/β-mediated nuclear import is critical for this feedback signaling, suggesting that proteins translocating between the cytoplasm and nucleus connect mRNA decay to transcription. We also show that an analogous pathway activated by vir

SUBMITTER: Duncan-Lewis C 

PROVIDER: S-EPMC8192121 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature