Clinical application of cell-free next-generation sequencing for infectious diseases at a tertiary children's hospital.

ABSTRACT:

Background

Children affected by infectious diseases may not always have a detectable infectious etiology. Diagnostic uncertainty can lead to prolonged hospitalizations, inappropriately broad or extended courses of antibiotics, invasive diagnostic procedures, and difficulty predicting the clinical course and outcome. Cell-free plasma next-generation sequencing (cfNGS) can identify viral, bacterial, and fungal infections by detecting pathogen DNA in peripheral blood. This testing modality offers the ability to test for many organisms at once in a shotgun metagenomic approach with a rapid turnaround time. We sought to compare the results of cfNGS to conventional diagnostic test results and describe the impact of cfNGS on clinical care in a diverse pediatric population at a large acade

SUBMITTER: Wilke J 

PROVIDER: S-EPMC8192220 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature