Ontology highlight
ABSTRACT:
SUBMITTER: Leibowitz ML
PROVIDER: S-EPMC8192433 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Leibowitz Mitchell L ML Papathanasiou Stamatis S Doerfler Phillip A PA Blaine Logan J LJ Sun Lili L Yao Yu Y Zhang Cheng-Zhong CZ Weiss Mitchell J MJ Pellman David D
Nature genetics 20210412 6
Genome editing has therapeutic potential for treating genetic diseases and cancer. However, the currently most practicable approaches rely on the generation of DNA double-strand breaks (DSBs), which can give rise to a poorly characterized spectrum of chromosome structural abnormalities. Here, using model cells and single-cell whole-genome sequencing, as well as by editing at a clinically relevant locus in clinically relevant cells, we show that CRISPR-Cas9 editing generates structural defects of ...[more]