Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine.

ABSTRACT: Fluorogenic RNA aptamers are synthetic functional RNAs that specifically bind and activate conditional fluorophores. The Chili RNA aptamer mimics large Stokes shift fluorescent proteins and exhibits high affinity for 3,5-dimethoxy-4-hydroxybenzylidene imidazolone (DMHBI) derivatives to elicit green or red fluorescence emission. Here, we elucidate the structural and mechanistic basis of fluorescence activation by crystallography and time-resolved optical spectroscopy. Two co-crystal structures of the Chili RNA with positively charged DMHBO⁺ and DMHBI⁺ ligands revealed a G-quadruplex and a trans-sugar-sugar edge G:G base pair that immobilize the ligand by π-π stacking. A Watson-Crick G:C base pair in the fluorophore binding site establishes a short hydrogen bond between

SUBMITTER: Mieczkowski M 

PROVIDER: S-EPMC8192780 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature