Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques.

ABSTRACT: Traditionally Caenorhabditis elegans lifespan assays are performed by manually inspecting nematodes with a dissection microscope, which involves daily counting of live/dead worms cultured in Petri plates for 21-25 days. This manual inspection requires the screening of hundreds of worms to ensure statistical robustness, and is therefore a time-consuming approach. In recent years, various automated artificial vision systems have been reported to increase the throughput, however they usually provide less accurate results than manual assays. The main problems identified when using these vision systems are the false positives and false negatives, which occur due to culture media changes, occluded zones, dirtiness or condensation of the Petri plates. In this work, we developed and described a ne

SUBMITTER: Puchalt JC 

PROVIDER: S-EPMC8192789 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature