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C. elegans survival assays to discern global and transcription-coupled nucleotide excision repair.

ABSTRACT: Global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER) protect cells against a variety of helix-distorting DNA lesions. In C. elegans, GG-NER primarily acts in proliferative germ cells and embryos, while TC-NER acts in post-mitotic somatic cells to maintain transcription. We leverage this difference to distinguish whether proteins function in GG-NER and/or TC-NER by straightforward UV survival assays. Here, we detail a protocol for these assays, using GG-NER factor xpc-1 and TC-NER factor csb-1 as examples. For complete details on the use and execution of this protocol, please refer to Sabatella et al. (2021).

SUBMITTER: van der Woude M

PROVIDER: S-EPMC8192855 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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<i>C. elegans</i> survival assays to discern global and transcription-coupled nucleotide excision repair.

van der Woude Melanie M Lans Hannes H

STAR protocols 20210608 2

Global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER) protect cells against a variety of helix-distorting DNA lesions. In <i>C. elegans</i>, GG-NER primarily acts in proliferative germ cells and embryos, while TC-NER acts in post-mitotic somatic cells to maintain transcription. We leverage this difference to distinguish whether proteins function in GG-NER and/or TC-NER by straightforward UV survival assays. Here, we detail a protocol for ...[more]

PMID: 34151304

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