CRISPR-Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains.

ABSTRACT: There is a demand to develop 3rd generation biorefineries that integrate energy production with the production of higher value chemicals from renewable feedstocks. Here, robust and stress-tolerant industrial strains of Saccharomyces cerevisiae will be suitable production organisms. However, their genetic manipulation is challenging, as they are usually diploid or polyploid. Therefore, there is a need to develop more efficient genetic engineering tools. We applied a CRISPR-Cas9 system for genome editing of different industrial strains, and show simultaneous disruption of two alleles of a gene in several unrelated strains with the efficiency ranging between 65% and 78%. We also achieved simultaneous disruption and knock-in of a reporter gene, and demonstrate the applicability of the m

SUBMITTER: Stovicek V 

PROVIDER: S-EPMC8193243 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature