Heterologous expression of MlcE in Saccharomyces cerevisiae provides resistance to natural and semi-synthetic statins.

ABSTRACT: Statins are inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase, the key enzyme in cholesterol biosynthesis. Their extensive use in treatment and prevention of cardiovascular diseases placed statins among the best selling drugs. Construction of Saccharomyces cerevisiae cell factory for the production of high concentrations of natural statins will require establishment of a non-destructive self-resistance mechanism to overcome the undesirable growth inhibition effects of statins. To establish active export of statins from yeast, and thereby detoxification, we integrated a putative efflux pump-encoding gene mlcE from the mevastatin-producing Penicillium citrinum into the S. cerevisiae genome. The resulting strain showed increased resistance to both natur

SUBMITTER: Ley A 

PROVIDER: S-EPMC8193252 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature