Refactoring of a synthetic raspberry ketone pathway with EcoFlex.

ABSTRACT:

Background

 A key focus of synthetic biology is to develop microbial or cell-free based biobased routes to value-added chemicals such as fragrances. Originally, we developed the EcoFlex system, a Golden Gate toolkit, to study genes/pathways flexibly using Escherichia coli heterologous expression. In this current work, we sought to use EcoFlex to optimise a synthetic raspberry ketone biosynthetic pathway. Raspberry ketone is a high-value (~ £20,000 kg^-1) fine chemical farmed from raspberry (Rubeus rubrum) fruit.

Results

 By applying a synthetic biology led design-build-test-learn cycle approach, we refactor the raspberry ketone pathway from a low level of productivity (0.2 mg/L), to achieve a 65-fold (12.9 mg/L) improvement in production. We perform this optimisati

SUBMITTER: Moore SJ 

PROVIDER: S-EPMC8193874 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature