Temporal induction of Lhx8 by optogenetic control system for efficient bone regeneration.

ABSTRACT:

Background

The spatiotemporal regulation of essential genes is crucial for controlling the growth and differentiation of cells in a precise manner during regeneration. Recently, optogenetics was considered as a potent technology for sophisticated regulation of target genes, which might be a promising tool for regenerative medicine. In this study, we used an optogenetic control system to precisely regulate the expression of Lhx8 to promote efficient bone regeneration.

Methods

Quantitative real-time PCR and western blotting were used to detect the expression of Lhx8 and osteogenic marker genes. Alkaline phosphatase staining and alizarin red staining were used to detect alkaline phosphatase activity and calcium nodules. A customized optogenetic expression system was constructed

SUBMITTER: Huang D 

PROVIDER: S-EPMC8194135 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature