SOX9 inactivation affects the proliferation and differentiation of human lung organoids.

ABSTRACT:

Background

The regulation of the transcription factor sex-determining region Y-box transcription factor 9 (SOX9) in lung development has been described in mouse, but the same principles apply to human lung development is unknown due to a lack of appropriate experimental approaches and models.

Methods

Here, we used gene editing technology to inactivate SOX9 in human embryonic stem cells that were then induced to differentiate into lung organoids to investigate the role of SOX9 in human lung epithelium development.

Results

Complete knockout of the transactivation domain of SOX9 by gene editing resulted in indels in both alleles of SOX9. SOX9^-/- hESCs could be induced to differentiate into lung progenitor organoids. In vitro long-term expansion showed that SOX

SUBMITTER: Li L 

PROVIDER: S-EPMC8194236 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature