Characterization of the gut microbiota in Chinese children with overweight and obesity using 16S rRNA gene sequencing.

ABSTRACT:

Background

Childhood obesity constitutes a worldwide health problem, and the gut microbiota play extremely important roles in obesity. Herein, we aimed to characterize the gut microbiota in children of normal weight, overweight, and obesity.

Methods

Thirty children of normal weight, 35 who were overweight, and 35 with obesity were enrolled from Nanjing, China. We isolated DNA from fecal samples, and employed 16S rRNA gene sequencing to explore the diversity and composition of gut microbiota.

Results

The operational taxonomic unit (OTU) numbers exhibited a reduction in the gut microbiota abundance with the increase in the body weight. Alpha diversity analysis revealed a sharp decrease in the mean microbial abundance among the three groups (Chao1: F = 5.478, P

SUBMITTER: Chen X 

PROVIDER: S-EPMC8194416 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature