Novel Canine Coronavirus Isolated from a Hospitalized Patient With Pneumonia in East Malaysia.

ABSTRACT:

Background

During the validation of a highly sensitive panspecies coronavirus (CoV) seminested reverse-transcription polymerase chain reaction (RT-PCR) assay, we found canine CoV (CCoV) RNA in nasopharyngeal swab samples from 8 of 301 patients (2.5%) hospitalized with pneumonia during 2017-2018 in Sarawak, Malaysia. Most patients were children living in rural areas with frequent exposure to domesticated animals and wildlife.

Methods

Specimens were further studied with universal and species-specific CoV and CCoV 1-step RT-PCR assays, and viral isolation was performed in A72 canine cells. Complete genome sequencing was conducted using the Sanger method.

Results

Two of 8 specimens contained sufficient amounts of CCoVs as confirmed by less-sensitive single-step RT-PCR ass

SUBMITTER: Vlasova AN 

PROVIDER: S-EPMC8194511 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature