Practical application of microsphere samples for benchmarking a quantitative phase imaging system.

ABSTRACT: Quantitative phase imaging (QPI) provides an approach for monitoring the dry mass of individual cells by measuring the optical pathlength of visible light as it passes through cells. A distinct advantage of QPI is that the measurements result in optical path length quantities that are, in principle, instrument independent. Reference materials that induce a well-defined optical pathlength shift and are compatible with QPI imaging systems will be valuable in assuring the accuracy of such measurements on different instruments. In this study, we evaluate seven combinations of microspheres embedded in index refraction matching media as candidate reference materials for benchmarking the performance of a QPI system and as calibration standards for the optical pathlength measurement. Poly(methyl m

SUBMITTER: Kwee E 

PROVIDER: S-EPMC8195315 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature