Technical framework for wastewater-based epidemiology of SARS-CoV-2.

ABSTRACT: Wastewater-based epidemiology (WBE) is expected to become a powerful tool to monitor the dissemination of SARS-CoV-2 at the community level, which has attracted the attention of scholars all over the world. However, there is not yet a standard protocol to guide its implementation. In this paper, we proposed a comprehensive technical and theoretical framework of relative quantification via qPCR for determining the virus abundance in wastewater and estimating the infection ratio in corresponding communities, which is expected to achieve horizontal and vertical comparability of the data using a human-specific biomarker as the internal reference. Critical factors affecting the virus detectability and the estimation of infection ratio include virus concentration methods, lag-period, per capita

SUBMITTER: Wu J 

PROVIDER: S-EPMC8195746 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature