Characterization of unique functionalities in c-Src domains required for osteoclast podosome belt formation.

ABSTRACT: Deletion of c-Src, a ubiquitously expressed tyrosine kinase, results in osteoclast dysfunction and osteopetrosis, in which bones harden into "stone." In contrast, deletion of the genes encoding other members of the Src family kinase (SFK) fails to produce an osteopetrotic phenotype. This suggests that c-Src performs a unique function in the osteoclast that cannot be compensated for by other SFKs. We aimed to identify the molecular basis of this unique role in osteoclasts and bone resorption. We found that c-Src, Lyn, and Fyn were the most highly expressed SFKs in WT osteoclasts, whereas Hck, Lck, Blk, and Fgr displayed low levels of expression. Formation of the podosome belt, clusters of unique actin assemblies, was disrupted in src^-/- osteoclasts; introduction of constitutively

SUBMITTER: Matsubara T 

PROVIDER: S-EPMC8196221 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature