Plant callus-derived shikimic acid regenerates human skin through converting human dermal fibroblasts into multipotent skin-derived precursor cells.

ABSTRACT:

Background

The human skin-derived precursors (SKPs) are a good cell source for regeneration. However, the isolation of SKP from human skin is limited. To overcome this drawback, we hypothesized that the component of plant stem cells could convert human fibroblasts to SKPs.

Methods

Human dermal fibroblasts were treated with shikimic acid, a major component of Sequoiadendron giganteum callus extract. The characteristics of these reprogrammed cells were analyzed by qPCR, western blot, colony-forming assay, and immunofluorescence staining. Artificial human skin was used for CO₂ laser-induced wound experiments. Human tissues were analyzed by immunohistochemistry.

Results

The reprogrammed cells expressed nestin (a neural precursor-specific protein), fibronectin,

SUBMITTER: Kwon YW 

PROVIDER: S-EPMC8196440 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature