D-dopachrome tautomerase activates COX2/PGE₂ pathway of astrocytes to mediate inflammation following spinal cord injury.

ABSTRACT:

Background

Astrocytes are the predominant glial cell type in the central nervous system (CNS) that can secrete various cytokines and chemokines mediating neuropathology in response to danger signals. D-dopachrome tautomerase (D-DT), a newly described cytokine and a close homolog of macrophage migration inhibitory factor (MIF) protein, has been revealed to share an overlapping function with MIF in some ways. However, its cellular distribution pattern and mediated astrocyte neuropathological function in the CNS remain unclear.

Methods

A contusion model of the rat spinal cord was established. The protein levels of D-DT and PGE₂ synthesis-related proteinase were assayed by Western blot and immunohistochemistry. Primary astrocytes were stimulated by different concentra

SUBMITTER: Ji H 

PROVIDER: S-EPMC8196514 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature