Physiological Characterization of Sulfolobus acidocaldarius in a Controlled Bioreactor Environment.

ABSTRACT: The crenarchaeal model organism Sulfolobus acidocaldarius is typically cultivated in shake flasks. Although shake flasks represent the state-of-the-art for the cultivation of this microorganism, in these systems crucial process parameters, like pH or substrate availability, are only set initially, but cannot be controlled during the cultivation process. As a result, a thorough characterization of growth parameters under controlled conditions is still missing for S. acidocaldarius. In this study, we conducted chemostat cultivations at 75 °C using a growth medium containing L-glutamate and D-glucose as main carbon sources. Different pH values and dilution rates were applied with the goal to physiologically characterize the organism in a controlled bioreactor environment. Under these controll

SUBMITTER: Rastadter K 

PROVIDER: S-EPMC8196767 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature