Biomolecular phase separation through the lens of sodium-23 NMR.

ABSTRACT: Phase separation is a fundamental physicochemical process underlying the spatial arrangement and coordination of cellular events. Detailed characterization of biomolecular phase separation requires experimental access to the internal environment of dilute and especially condensed phases at high resolution. In this study, we take advantage from the ubiquitous presence of sodium ions in biomolecular samples and present the potentials of ²³ Na NMR as a proxy to report the internal fluidity of biomolecular condensed phases. After establishing the temperature and viscosity dependence of ²³ Na NMR relaxation rates and translational diffusion coefficient, we demonstrate that ²³ Na NMR probes of rotational and translational mobility of sodium ions are capable of ca

SUBMITTER: Fuentes-Monteverde JC 

PROVIDER: S-EPMC8197435 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature