S1P Lyase Regulates Intestinal Stem Cell Quiescence via Ki-67 and FOXO3.

ABSTRACT:

Background

Reduction of the Sphingosine-1-phosphate (S1P) degrading enzyme S1P lyase 1 (SGPL1) initiates colorectal cancer progression with parallel loss of colon function in mice. We aimed to investigate the effect of SGPL1 knockout on the stem cell niche in these mice.

Methods

We performed immunohistochemical and multi-fluorescence imaging on tissue sections of wildtype and SGPL1 knockout colons under disease conditions. Furthermore, we generated SGPL1 knockout DLD-1 cells (SGPL1^-/-M.Ex1) using CRISPR/Cas9 and characterized cell cycle and AKT signaling pathway via Western blot, immunofluorescence, and FACS analysis.

Results

SGPL1 knockout mice were absent of anti-Ki-67 staining in the stem cell niche under disease conditions. This was accompanied by an in

SUBMITTER: Schwiebs A 

PROVIDER: S-EPMC8198365 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature