The Role of Interleukin-1-Receptor-Antagonist in Bladder Cancer Cell Migration and Invasion.

ABSTRACT:

Background

The interleukin-1-receptor antagonist IL1RA (encoded by the IL1RN gene) is a potent competitive antagonist to interleukin-1 (IL1) and thereby is mainly involved in the regulation of inflammation. Previous data indicated a role of IL1RA in muscle-invasive urothelial carcinoma of the bladder (UCB) as well as an IL1-dependent decrease in tissue barrier function, potentially contributing to cancer cell invasion.

Objective

Based on these observations, here we investigated the potential roles of IL1RA, IL1A, and IL1B in bladder cancer cell invasion in vitro.

Methods

Cell culture, real-time impedance sensing, invasion assays (Boyden chamber, pig bladder model), qPCR, Western blot, ELISA, gene overexpression.

Results

We observed a loss of IL1RA expres

SUBMITTER: Schneider L 

PROVIDER: S-EPMC8198563 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature