Multitargeted Approach for the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents.

ABSTRACT: In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions. Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects. However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised. Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR). In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches. Th

SUBMITTER: Mieremet A 

PROVIDER: S-EPMC8198964 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature